ProSci

ASRGL1 Antibody [CRASH/1289]

Product Code:
 
PSI-33-605
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CRASH/1289
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
2 / 4
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4 / 4
SDS-PAGE Analysis of Purified, BSA-Free ASRGL1 Antibody (clone CRASH/1289). Confirmation of Integrity and Purity of the Antibody.
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IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289). Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.
SDS-PAGE Analysis of Purified, BSA-Free ASRGL1 Antibody (clone CRASH/1289). Confirmation of Integrity and Purity of the Antibody.

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PSI-33-605-100ug100ug£765.00
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Further Information

Additional Names:
ASRGL1, asparaginase like 1, ALP, ALP1, FLJ22316, asparaginase-like 1 protein, CRASH
Application Note:
Western blot: 0.5-1 ug/ml

Flow Cytometry: 0.5-1ug/10^6 cells in 0.1ml

IF: 0.5-1 ug/ml

IHC (FFPE): 0.5-1 ug/ml

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ASRGL1 antibody to be titered up or down for optimal performance.
Background:
ASRGL1 (Asparaginase-like protein 1), also known as CRASH, belongs to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. It is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A human partial recombinant protein was used as the immunogen for this ASRGL1 antibody.
NCBI Gene ID #:
80150
NCBI Official Name:
asparaginase like 1
NCBI Official Symbol:
ASRGL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G affinity chromatography
Research Area:
Obesity, Neuroscience, Signal Transduction
Swissprot #:
Q7L266
User NOte:
Optimal dilutions for each application to be determined by the researcher