ProSci

Bax Antibody [2D2]

Product Code:
 
PSI-99-622
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
[2D2]
Regulatory Status:
 
RUO
Target Species:
  • Bovine (Cattle)
  • Canine (dog)
  • Human
  • Monkey
Application:
 
Flow Cytometry
Shipping:
 
blue ice
Storage:
 
Store vial at 2-8˚C
1 / 1
Total cell lysates from HL60 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bax-UNLB (Cat. No. 99-622). Proteins were visualized using Goat Anti-Mouse IgG1, Human ads-HRP secondary antibody and chemiluminescent detection.
❮❯

Total cell lysates from HL60 cells were resolved by electrophoresis, transferred to PVDF membrane, and probed with Mouse Anti-Human Bax-UNLB (Cat. No. 99-622). Proteins were visualized using Goat Anti-Mouse IgG1, Human ads-HRP secondary antibody and chemiluminescent detection.

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PSI-99-622-0.1mg0.1mg£505.00
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Further Information

Additional Names:
Apoptosis regulator BAX, BCL2 associated X protein
Application Note:
Bax Antibody [2D2] for use in flow cytometry, immunohistochemistry / immunocytochemistry, western blotting, immunoprecipitation, purification, and neutralization assays.
Background:
Bax is a 20-22 kDa member of the Bcl-2 family of proteins involved in regulation of programmed cell death, or apoptosis. In murine thymocytes, it is expressed primarily as a cytoplasmic protein. Over-expression of Bax promotes apoptosis by formation of homodimers and through heterodimerization with Bcl-2, an inhibitor of apoptosis. The monoclonal antibody 2D2 reacts with an N-terminal epitope between amino acids 3-16 of human Bax. It can bind both soluble and detergent-altered forms of Bax as well as Bax-Bcl-2 and Bax-Bcl-xL heterodimers.
Buffer:
Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added
Concentration:
0.1 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human Bax peptide (a.a. 3-16)
NCBI Gene ID #:
581
NCBI Official Name:
BCL2-associated X protein
NCBI Official Symbol:
BAX
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
Q07812
Research Area:
Membrane,Cell Cycle,Cancer,Apoptosis
SPECIFICITY:
Bax
Swissprot #:
Q07812
User NOte:
Optimal dilutions for each application to be determined by the researcher.