ProSci

Recombinant CD31 Antibody [C31/1395R]

Product Code:
 
PSI-34-062
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG, k
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
C31/1395R
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: steam sections in pH7.5-8.5, 1mM EDTA for 10-20 min.
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IHC staining of FFPE human angiocarcinoma with recombinant CD31 antibody (clone C31/1395R). Required HIER: steam sections in pH7.5-8.5, 1mM EDTA for 10-20 min.

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PSI-34-062-100ug100ug£765.00
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Further Information

Additional Names:
Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
Application Note:
Immunofluorescence: 0.5-1 ug/ml

Flow Cytometry: 0.5-1ug/10e6 cells in 0.1ml

Immunohistochemistry (FFPE): 1-2 ug/ml for 30 minutes at RT

Prediluted IHC only format: incubate for 30 min at RT (1)

Titering of the recombinant CD31 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Background:
CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.2 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Human full length recombinant protein was used as the immunogen for the recombinant CD31 antibody.
NCBI Gene ID #:
5175
NCBI Official Name:
platelet/endothelial cell adhesion molecule 1
NCBI Official Symbol:
PECAM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein A affinity
Research Area:
Cancer,Immunology,Signal Transduction
Swissprot #:
P16284
User NOte:
Optimal dilutions for each application to be determined by the researcher