ProSci

PD1 Antibody [7H6] (biotin)

Product Code:
 
PSI-RF16003-biotin
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
7H6
Regulatory Status:
 
RUO
Target Species:
 
Human
Shipping:
 
blue ice
Storage:
 
PD-1 Antibody (biotin) can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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A sandwich ELISA was performed using the anti-PD-1 mAb RF16006 (5 μg/ml) as the capture antibody. Biotin-labeled anti-PD-1 mAb RF16003-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.
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A sandwich ELISA was performed using the anti-PD-1 mAb RF16006 (5 μg/ml) as the capture antibody. Biotin-labeled anti-PD-1 mAb RF16003-biotin (1 μg/ml) and streptavidin-HRP (0.1 μg/ml) were used for detection. Detection range is from 10 ng to 40 pg.

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CodeSizePrice
PSI-RF16003-biotin-0.02mg0.02mg£150.00
Quantity:
PSI-RF16003-biotin-0.1mg0.1mg£515.00
Quantity:
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Further Information

Additional Names:
PD-1 Antibody: PD1, PD-1, CD279, SLEB2, hPD-1, hPD-l, hSLE1, PD1, Programmed cell death protein 1, Protein PD-1, PDCD1, PDCD-1
Application Note:
PD1 antibody can be used for as detection antibody in Sandwich Elisa at 1 μg/mL.
Background:
Cell-mediated immune responses are initiated by T lymphocytes that are themselves stimulated by cognate peptides bound to MHC molecules on antigen-presenting cells (APC). T-cell activation is generally self-limited as activated T cells express receptors such as PD-1 (also known as PDCD-1) that mediate inhibitory signals from the APC. PD-1 can bind two different but related ligands, PDL-1 and PDL-2. Upon binding to either of these ligands, signals generated by PD-1 inhibit the activation of the immune response in the absence of "danger signals" such as LPS or other molecules associated with bacteria or other pathogens. Evidence for this is seen in PD-1-null mice who exhibit hyperactivated immune systems and autoimmune diseases. PD-1 is thus one of a growing number of immune checkpoint proteins.
Background References:
  • Holling TM, Schooten E, and van Den Elsing PJ. Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men. Hum. Immunol. 2004; 65:282-90.
  • Ishida Y, Agata Y, Shibahara K, et al. Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J. 1992; 11:3887-95.
  • Zhong X, Bai C, Gao W, et al. Suppression of expression and function of negative immune regulator PD-1 by certain pattern recognition and cytokine receptor signals associated with immune system danger. Int. Immunol. 2004; 16:1181-8.
  • Nishimura H, Nose M, Hiai H, et al. Development of lupus-like autoimmune diseases by the disruption of the PD-1 gene encoding an ITIM motif-carrying immunoreceptor. Immunity 1999; 11:141-51.
Buffer:
PD-1 Antibody (biotin) is supplied in PBS containing 1% BSA and 0.02% sodium azide.
Concentration:
1 mg/mL
Conjugate:
biotin
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
PD-1 antibody (biotin) was raised against the extracellular domain of human PD-1.
NCBI Gene ID #:
5133
NCBI Official Name:
programmed cell death 1
NCBI Official Symbol:
PDCD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Protein Accession #:
NP_005009
Protein GI Number:
145559515
Purification:
PD-1 Antibody (biotin) is supplied as protein A purified IgG1.
Research Area:
Immunology
Swissprot #:
Q15116
User NOte:
Optimal dilutions for each application to be determined by the researcher.