ProSci

GAPDH Antibody [GAP259]

Product Code:
 
PSI-33-017
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
GAP259
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Application:
 
Western Blot (WB)
Shipping:
 
blue ice
Storage:
 
Aliquot and Store at -20C. Avoid freez-thaw cycles.
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Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.
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IHC testing of FFPE human liver tissue with GAPDH antibody at 3ug/ml.
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Western blot testing of human samples with GAPDH antibody at 0.5ug/ml.
IHC testing of FFPE human liver tissue with GAPDH antibody at 3ug/ml.

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CodeSizePrice
PSI-33-017-100ug100ug£662.00
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Further Information

Additional Names:
GAPDH Antibody: Glyceraldehyde-3-phosphate dehydrogenase, G3PDH, GAPD
Application Note:
Western blot: 0.5-1 ug/ml

Provided assay concentrations are suggestions only, GAPDH antibody titration may be required for optimal results.
Background:
Due to its ubiquitous presence in mammalian cells, GAPDH is often used a loading control protein in western blot. GAPDH antibody is used to control for/demonstrate equivalent protein loading in different gel lanes, typically transfected vs mock or non transfected cell lysate.
Buffer:
PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration:
0.5 mg/mL
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A recombinant protein fragment from C-terminal GAPDH was used as the immunogen.
NCBI Gene ID #:
2597
NCBI Official Name:
glyceraldehyde-3-phosphate dehydrogenase
NCBI Official Symbol:
GAPDH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
Purification:
Protein G Affinity Chromatography
Research Area:
Signal Transduction,Neuroscience
Swissprot #:
P04406
User NOte:
Optimal dilutions for each application to be determined by the researcher