South Bay Bio

20S Immunoproteasome Assay Kit

Product Code:
 
SBB-KP0037
Supplier:
 
South Bay Bio
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Compound Screening
Shipping:
 
DRY ICE
Storage:
 
-20°C
1 / 1
% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit
❮❯

% Signal to Background of Continuous Real-Time TR-FRET Parkin titration (autoubiquitination): Serial dilutions of Parkin W403A from 50nM to 3.125nM and 300nM wt Parkin were mixed with UBA1, UBE2D3 and trf-Ub (pS65) mix. Reactions were initiated wit

No additional charges, what you see is what you pay! *

CodeSizePrice
SBB-KP0037-KI011 Kit£717.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: Switzerland.
Typical lead time: 10-14 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Detection Type:
Fluorescent
EClass:
32160000
Handling Advice:
Aliquot to avoid freeze/thaw cycles.Avoid freeze/thaw cycles.
Kit Contains:
10x 20S Immunoproteasome | 50x LLVY-AMC, Chemotrypsin-like activity | 50x PAL-AMC, beta1i/PSMB9 specific substrate | 50x ANW-AMC, beta5i/PSMB8 specific substrate | 50x Inhibitor (ONX-0914, 2mM) in 100% DMSO | 10x Reaction Buffer | 50x SDS(1.75%) in H20 | 100x free AMC Standard (40µM)
Long Description:
South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
Package Type:
Vial
Product Description:
South Bay Bio's kit is designed to test for specific activity of 20S immunoproteasome. The kit provides purified 20S immunoproteasome and is designed to test for Chymotrypsin-like activity (Suc-LLVY-AMC) and Caspase-like activity of the immunoproteasome subunits beta1i/ PSMB9 (PAL-AMC) and beta5i/PSMB8 (ANW-AMC). Additionally, the compound, ONX-0914, which can be used to inhibit specifically the subunit beta5i/LMP7 20S immunoproteasome is included. All peptide substrates are conjugated to AMC, which upon proteasome catalyzed hydrolyses display fluorescence at Excitation=345nm, Emission= 445nm; allowing for a real-time read out of 20S immunoproteasome specific activity.
Specificity:
Readout: Endpoint/Kinetic Label or Dye: 7-Amino-4-methylcoumarin Detection Method: Fluorescence Substrate Properties: Protein-based Substrate. Excitation/Emission (nm): 345/445
Transportation:
Non-hazardous
UNSPSC Category:
Chemistry test kits or supplies
UNSPSC Number:
41116104