Antibody Clonality:
Monoclonal
Target Species:- Chicken (Gallus)
- Human
- Mouse
- Rat
Applications:- Enzyme-Linked Immunosorbent Assay (ELISA)
- Immunofluorescence (IF)
- Immunohistochemistry- Frozen Section (IHC-F)
- Immunohistochemistry- Paraffin Embedded (IHC-P)
- Immunoprecipitation (IP)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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This product comes from:
Netherlands.
Typical lead time:
7-10 working days.
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Further Information
Applications Description:
This antibody can be used for electron microscopy, ELISA, immunofluorescence, immunoprecipitation (2 ?g/mg of protein lysate),and immunohistochemistry on frozen and formalin/paraffin fixed tissues (2-4 ?g/ml). Optimal concentration should be evaluated by serial dilutions.
Recognizes a protein of 45kDa, identified as MyoD1. The epitope of this antibody maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Occassionally nuclear expression/staining of MyoD1 is seen in ectomesenchymoma and a subset of Wilm?s tumors. Weak cytoplasmic staining/presence is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing?s sarcomas and alveolar soft part sarcomas. The 5.2F antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm?s tumors.
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
See vial for concentration
Signal Transduction
Provided as solution in phosphate buffered saline with 0.08% sodium azide
Western Blotting
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with recombinant mouse MyoD1 protein and mouse myeloma Sp2/0-Ag14 cells.
Rhabdomyosarcoma, SW80 cells
Unconjugated
Products are stable for one year from purchase when stored properly
Protein A/G Chromatography
Myoblast determination protein 1, Myogenic factor 3, MyoD1, Myf-3
P15172 (Human)P10085 (Mouse)Q02346 (Rat)P16075 (Chicken)