Antibody Clonality:
Monoclonal
Target Species:
Species Independent
Application:
Western Blot (WB)
Shipping:
Ship at ambient temperature freeze upon arrival
Storage:
Product should be stored at -20°C. Aliquot to avoid freeze/thaw cycles
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This product comes from:
Netherlands.
Typical lead time:
7-10 working days.
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Further Information
Applications Description:
Antibody can be used for Western blotting (Suggested dilution - 1:1000) and immunohistochemistry. Optimal concentration should be evaluated by serial dilutions.
Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxidesignaling. While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase, myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Various studies have shown that protein tyrosinenitration is limited to specific proteins and that the process is selective. For example, exposure of human surfactant protein A (SP-A) to oxygen-nitrogen intermediates generated by activated alveolar macrophages resulted in specific nitration of SP-A at tyrosines 164 and 166, while addition of 1.2 mMCO 2 resulted in additional nitration at tyrosine 161. The presence of nitrotyrosine-containing proteins has shown high correlation to disease states such as atherosclerosis, Alzheimer?s disease, Parkinson?s disease and amyotrophic lateral sclerosis.55 kD 160 kD
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals.
See vial for concentration
Signal Transduction
20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3mM sodium azide, pH 7.5
Western Blotting
3-Nitrotyrosine-KLH
100 ug monoclonal antibody in 20 mM sodium phosphate, 150 mM sodium chloride, 50% glycerol, 3mM sodium azide, pH 7.5
Unconjugated
Products are stable for one year from purchase when stored properly
Mouse monoclonal antibody 2A12, obtained after immunization of mice with 3-Nitrotyrosine-KLH
Nitrotyrosine