NSJ Bioreagents

PAK1/2/3 Antibody (pS144/pS141/pS139)

Product Code:
 
NSJ-RQ4789
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
CFF-16
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
Applications:
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the phospho-PAK1/2/3 antibody at -20°C.
1 / 2
Western blot testing of lysate from lambda phosphatase-treated human HeLa cells with phospho-PAK1/2/3 antibody. Predicted molecular weight ~62 kDa.
2 / 2
IHC staining of FFPE mouse brain with phospho-PAK1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
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Western blot testing of lysate from lambda phosphatase-treated human HeLa cells with phospho-PAK1/2/3 antibody. Predicted molecular weight ~62 kDa.
IHC staining of FFPE mouse brain with phospho-PAK1/2/3 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.

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NSJ-RQ4789-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:1000-1:10000,Immunohistochemistry (FFPE): 1:50-1:200
Application Note:
Optimal dilution of the phospho-PAK1/2/3 antibody should be determined by the researcher.
Description:
PAK proteins, a family of serine/threonine p21-activating kinases, serve as targets for the small GTP binding proteins Cdc42 and RAC and have been implicated in a wide range of biological activities.
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human PAK1/2/3 (surrounding pS144/pS141/pS139) was used as the immunogen for the phospho-PAK1/2/3 antibody.
Limitation:
This phospho-PAK1/2/3 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human, Mouse
Uniprot #:
O75914