NSJ Bioreagents

HER2 Antibody / ErbB2

Product Code:
 
NSJ-RQ5055
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
BCI-5
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunocytochemistry (ICC)
  • Immunofluorescence (IF)
  • Immunohistochemistry (IHC)
  • Western Blot (WB)
Storage:
 
Store the HER2 antibody at -20°C.
1 / 3
IF/ICC staining of human SK-BR-3 cells with HER2 antibody (green) and DAPI nuclear stain (blue).
2 / 3
IHC staining of FFPE human breast carcinoma with HER2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
3 / 3
Western blot testing of human SK-BR-3 cell lysate with HER2 antibody.
❮❯

IF/ICC staining of human SK-BR-3 cells with HER2 antibody (green) and DAPI nuclear stain (blue).
IHC staining of FFPE human breast carcinoma with HER2 antibody. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min and allow to cool before testing.
Western blot testing of human SK-BR-3 cell lysate with HER2 antibody.

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NSJ-RQ5055-100ul100ul£535.00
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Further Information

Application Details :
Western blot: 1:500-1:2000,Immunohistochemistry (FFPE): 1:50-1:200,Immunofluorescence/Immunocytochemistry: 1:50-1:200
Application Note:
Optimal dilution of the HER2 antibody should be determined by the researcher.
Description:
The ERBB2 gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. [RefSeq]
Format :
Purified
Formulation :
Antibody in PBS with 0.02% sodium azide and 50% glycerol
Immunogen:
A synthetic peptide specific to human HER2 / ErbB2 was used as the immunogen for the HER2 antibody.
Limitation:
This HER2 antibody is available for research use only.
Purity:
Affinity purified
Species Reactivity :
Human
Uniprot #:
P04626