DL-Dithiothreitol
Product Code:
CDX-D0323
CDX-D0323
Antibody Isotype:
n/a
n/a
Antibody Clone:
n/a
n/a
Regulatory Status:
RUO
RUO
Shipping:
Ambient
Ambient
Storage:
+4°C
+4°C
No additional charges, what you see is what you pay! *
| Code | Size | Price |
|---|
| CDX-D0323-G005 | 5 g | £96.00 |
Quantity:
| CDX-D0323-G010 | 10 g | £151.00 |
Quantity:
| CDX-D0323-G050 | 50 g | £572.00 |
Quantity:
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This product comes from: Switzerland.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
Typical lead time: 7-10 working days.
Contact us for more accurate information.
- Further Information
- Documents
- References
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Further Information
Alternate Names/Synonyms:
threo-1,4-Dimercapto-2,3-butanediol; Cleland?s reagent; DTT
Appearance:
White powder.
CAS:
03/12/3483
Class:
9
EClass:
32160000
Form (Short):
liquid
GHS Symbol:
GHS07
Handling Advice:
Protect from light and moisture.
Hazards:
H302-H315-H319-H335
InChi:
InChI=1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m0/s1
InChiKey:
VHJLVAABSRFDPM-IMJSIDKUSA-N
Long Description:
Chemical. CAS: 3483-12-3. Formula: C4H10O2S. MW: 154.25. DTT is a reducing agent, which once oxidized forms a stable six-membered ring with an internal disulfide bond. It is an excellent reagent for maintaining SH groups in reduced state. DTT is used as a protecting agent that prevents oxidation of thiol groups, and used as a reducing agent for thiolated DNA. DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. DTT is often used along with sodium dodecylsulfate in SDS-PAGE to further denature proteins by reducing their disulfide bonds to allow for better separation of proteins during electrophoresis. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione.
MDL:
MFCD00004877
Molecular Formula:
C4H10O2S
Molecular Weight:
154.25
Package Type:
Vial
PG:
III
Precautions:
P261-P305 + P351 + P338
Product Description:
DTT is a reducing agent, which once oxidized forms a stable six-membered ring with an internal disulfide bond. It is an excellent reagent for maintaining SH groups in reduced state. DTT is used as a protecting agent that prevents oxidation of thiol groups, and used as a reducing agent for thiolated DNA. DTT is frequently used to reduce the disulfide bonds of proteins and, more generally, to prevent intramolecular and intermolecular disulfide bonds from forming between cysteine residues of proteins. DTT is often used along with sodium dodecylsulfate in SDS-PAGE to further denature proteins by reducing their disulfide bonds to allow for better separation of proteins during electrophoresis. DTT can also be used as an oxidizing agent. Its principal advantage is that effectively no mixed-disulfide species are populated, in contrast to other agents such as glutathione.
Purity:
>98 (AT)
Signal word:
Warning
SMILES:
[H][C@@](O)([C@](O)([H])CS)CS
Solubility Chemicals:
Soluble in water (50mg/ml).
Source / Host:
Synthetic.
Transportation:
Excepted Quantity
UN Nummer:
3335
UNSPSC Category:
Biochemical Reagents
UNSPSC Number:
12352200
Use & Stability:
Stable for at least 2 years after receipt when stored at +4°C.
Documents
References
(1) W.W. Cleland; Biochemistry 3, 480 (1964) | (2) U.T. Ruegg & J. Rudinger; Methods Enzymol. 47, 111 (1977) | (3) D.W. Sears, et al; Biochemistry 16, 2031 (1977) | (4) M.C. Alliegro; Anal. Biochem. 282, 102 (2000) | (5) A.C. Grabski & R.R. Burgess; inNovations 13, 10 (2001)