ProSci

Perforin Antibody

Product Code:
 
PSI-13-029
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using Perforin antibody (13-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody (13-029) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody (13-029) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using Perforin antibody (13-029). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using Perforin antibody (13-029) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded mouse spleen using Perforin antibody (13-029) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using Perforin antibody (13-029) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using Perforin antibody (13-029). Blue: DAPI for nuclear staining.

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PSI-13-029-50uL50uL£433.00
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Further Information

Additional Names:
PFP, Perforin-1, Cytolysin, P1, PFP, FLH2, PFN1, HPLH2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. This protein is one of the main cytolytic proteins of cytolytic granules, and it is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants encoding the same protein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 280-555 of human Perforin (NP_001076585.1).
NCBI Gene ID #:
5551
NCBI Official Name:
perforin 1 (pore forming protein)
NCBI Official Symbol:
PRF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 70kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Innate Immunity
Swissprot #:
P14222
User NOte:
Optimal dilutions for each application to be determined by the researcher.