ProSci

CD31 Antibody

Product Code:
 
PSI-13-159
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of mouse liver, using CD31 antibody (13-159) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 4
Immunofluorescence analysis of U2OS cells using CD31 antibody (13-159) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of HUVEC cells using CD31 antibody (13-159) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of human placenta cells using CD31 antibody (13-159) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse liver, using CD31 antibody (13-159) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using CD31 antibody (13-159) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HUVEC cells using CD31 antibody (13-159) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human placenta cells using CD31 antibody (13-159) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-13-159-50uL50uL£433.00
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Further Information

Additional Names:
Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam, Pecam-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31 (NP_001292086.1).
NCBI Gene ID #:
18613
NCBI Official Name:
Platelet endothelial cell adhesion molecule
NCBI Official Symbol:
PECAM1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P16284
User NOte:
Optimal dilutions for each application to be determined by the researcher.