ProSci

MTHFD2 Antibody

Product Code:
 
PSI-13-620
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (13-620) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human mammary cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH-3T3 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MTHFD2 antibody (13-620) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human mammary cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using MTHFD2 antibody (13-620) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using MTHFD2 antibody (13-620) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-13-620-50uL50uL£433.00
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Further Information

Additional Names:
MTHFD2, NMDMC
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-250 of human MTHFD2 (NP_006627.2).
NCBI Gene ID #:
10797
NCBI Official Name:
methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
NCBI Official Symbol:
MTHFD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P13995
User NOte:
Optimal dilutions for each application to be determined by the researcher.