ProSci

UBR4 Antibody

Product Code:
 
PSI-14-306
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of HeLa cells, using UBR4 antibody (14-306) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of HeLa cells, using UBR4 antibody (14-306) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

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PSI-14-306-50uL50uL£433.00
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Further Information

Additional Names:
UBR4, zinc finger, UBR1 type 1, retinoblastoma-associated factor 600-like protein, retinoblastoma-associated factor 600, p600, ZUBR1, RBAF600, KIAA1307, KIAA0462, FLJ41863, ubiquitin protein ligase E3 component n-recognin 4, UBR4
Application Note:
WB: 1:500 - 1:1000
Background:
The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus. In addition, this protein is a target of the human papillomavirus type 16 E7 oncoprotein.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 4884-5183 of human UBR4 (NP_065816.2).
NCBI Gene ID #:
23352
NCBI Official Name:
ubiquitin protein ligase E3 component n-recognin 4
NCBI Official Symbol:
UBR4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 600kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q5T4S7
User NOte:
Optimal dilutions for each application to be determined by the researcher.