ProSci

CD40LG Antibody

Product Code:
 
PSI-14-739
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of K561 cells, using CD40LG antibody (14-739) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 4
Immunofluorescence analysis of HeLa cells using CD40LG antibody (14-739) at dilution of 1:100. Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of NIH/3T3 cells using CD40LG antibody (14-739) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of PC-12 cells using CD40LG antibody (14-739) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of K561 cells, using CD40LG antibody (14-739) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of HeLa cells using CD40LG antibody (14-739) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CD40LG antibody (14-739) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC-12 cells using CD40LG antibody (14-739) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-14-739-50uL50uL£433.00
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Further Information

Additional Names:
CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD154, CD40 ligand, membrane form, CD40 ligand, soluble form, CD40LG, CD40L, TNFSF5, TRAP
Application Note:
WB: 1:1000 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 79-129 of human CD40LG (NP_000065.1).
NCBI Gene ID #:
959
NCBI Official Name:
CD40 ligand
NCBI Official Symbol:
CD40LG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 29kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Chemokines & Cytokines, Immunology, Innate Immunity
Swissprot #:
P29965
User NOte:
Optimal dilutions for each application to be determined by the researcher.