ProSci

PHF11 Antibody

Product Code:
 
PSI-14-982
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of SW480 cells, using PHF11 antibody (14-982) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 3
Immunohistochemistry of paraffin-embedded mouse lung using PHF11 antibody (14-982) at dilution of 1:100 (40x lens).
3 / 3
Immunofluorescence analysis of A549 cells using PHF11 antibody (14-982).

Western blot analysis of extracts of SW480 cells, using PHF11 antibody (14-982) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using PHF11 antibody (14-982) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PHF11 antibody (14-982).

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PSI-14-982-50uL50uL£433.00
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This product comes from: United States.
Typical lead time: 14-21 working days.
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Further Information

Additional Names:
PHF11, APY, BCAP, IGEL, IGER, IGHER, NY-REN-34, NYREN34, RP11-185C18.3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a protein containing a PHD (plant homeodomain) type zinc finger. This gene has been identified in some studies as a candidate gene for asthma. Naturally-occurring readthrough transcription may occur from the upstream SETDB2 (SET domain bifurcated 2) gene to this locus. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human PHF11 (NP_001035533.1).
NCBI Gene ID #:
51131
NCBI Official Name:
PHD finger protein 11
NCBI Official Symbol:
PHF11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 34kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
Q9UIL8
User NOte:
Optimal dilutions for each application to be determined by the researcher.