ProSci

RAD18 Antibody

Product Code:
 
PSI-14-988
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using RAD18 antibody (14-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
2 / 3
Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
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Western blot analysis of extracts of various cell lines, using RAD18 antibody (14-988) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 120s.
Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using RAD18 antibody (14-988). Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.

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PSI-14-988-50uL50uL£433.00
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Further Information

Additional Names:
RAD18, RNF73
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 216-495 of human RAD18 (NP_064550.3).
NCBI Gene ID #:
56852
NCBI Official Name:
RAD18 homolog (S. cerevisiae)
NCBI Official Symbol:
RAD18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9NS91
User NOte:
Optimal dilutions for each application to be determined by the researcher.