ProSci

LSM4 Antibody

Product Code:
 
PSI-15-090
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using LSM4 antibody (15-090) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug LSM4 antibody (15-090). Western blot was performed from the immunoprecipitate using LSM4 antibody (15-090) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using LSM4 antibody (15-090) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells using 1ug LSM4 antibody (15-090). Western blot was performed from the immunoprecipitate using LSM4 antibody (15-090) at a dilition of 1:1000.

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PSI-15-090-50uL50uL£433.00
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Further Information

Additional Names:
LSM4, YER112W, GRP
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a member of the LSm family of RNA-binding proteins. LSm proteins form stable heteromers that bind specifically to the 3'-terminal oligo(U) tract of U6 snRNA and may play a role in pre-mRNA splicing by mediating U4/U6 snRNP formation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-139 of human LSM4 (NP_036453.1).
NCBI Gene ID #:
25804
NCBI Official Name:
LSM4 homolog, U6 small nuclear RNA associated (S. cerevisiae)
NCBI Official Symbol:
LSM4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 14kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9Y4Z0
User NOte:
Optimal dilutions for each application to be determined by the researcher.