ProSci

CCAR1 Antibody

Product Code:
 
PSI-15-094
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using CCAR1 antibody (15-094) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 5s.
2 / 3
Immunofluorescence analysis of HeLa cells using CCAR1 antibody (15-094). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CCAR1 antibody (15-094). Western blot was performed from the immunoprecipitate using CCAR1 antibody (15-094) at a dilition of 1:500.
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Western blot analysis of extracts of various cell lines, using CCAR1 antibody (15-094) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Basic Kit._Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using CCAR1 antibody (15-094). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug CCAR1 antibody (15-094). Western blot was performed from the immunoprecipitate using CCAR1 antibody (15-094) at a dilition of 1:500.

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PSI-15-094-50uL50uL£433.00
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Further Information

Additional Names:
CCAR1, cell-cycle and apoptosis regulatory protein 1, OTTHUMP00000019709, RP11-437A18.1, MGC44628, CARP1, CARP-1, cell division cycle and apoptosis regulator 1, CCAR1, DIS
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human CCAR1 (NP_060707.2).
NCBI Gene ID #:
55749
NCBI Official Name:
cell division cycle and apoptosis regulator 1
NCBI Official Symbol:
CCAR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 155kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q8IX12
User NOte:
Optimal dilutions for each application to be determined by the researcher.