ProSci

LIMS1 Antibody

Product Code:
 
PSI-15-265
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of mouse heart, using LIMS1 antibody (15-265) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of A549 cells using LIMS1 antibody (15-265). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse heart, using LIMS1 antibody (15-265) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using LIMS1 antibody (15-265). Blue: DAPI for nuclear staining.

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PSI-15-265-50uL50uL£433.00
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Further Information

Additional Names:
PINCH, PINCH-1, PINCH1, LIM and senescent cell antigen-like-containing domain protein 1, LIM and senescent cell antigen-like domains 1, LIM-type zinc finger domains 1, particularly interesting new Cys-His protein 1, renal carcinoma antigen NY-REN-48, senescent cell antigen
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-325 of human LIMS1 (NP_001180417.1).
NCBI Gene ID #:
3987
NCBI Official Name:
LIM zinc finger domain containing 1
NCBI Official Symbol:
LIMS1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors, Signal Transduction
Swissprot #:
P48059
User NOte:
Optimal dilutions for each application to be determined by the researcher.