ProSci

COMP Antibody

Product Code:
 
PSI-15-297
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using COMP antibody (15-297) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 3
Immunohistochemistry of paraffin-embedded rat trachea using COMP antibody (15-297) at dilution of 1:100 (40x lens).
3 / 3
Immunohistochemistry of paraffin-embedded human liver using COMP antibody (15-297) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using COMP antibody (15-297) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat trachea using COMP antibody (15-297) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using COMP antibody (15-297) at dilution of 1:100 (40x lens).

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PSI-15-297-50uL50uL£433.00
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Further Information

Additional Names:
EDM1, EPD1, MED, PSACH, THBS5, TSP5, cartilage oligomeric matrix protein, cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple), pseudoachondroplasia (epiphyseal dysplasia 1, multiple), thrombospondin-5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Contraction or expansion of a 5 aa aspartate repeat and other mutations can cause pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED).
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-160 of human COMP (NP_000086.2).
NCBI Gene ID #:
1311
NCBI Official Name:
cartilage oligomeric matrix protein
NCBI Official Symbol:
COMP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
P49747
User NOte:
Optimal dilutions for each application to be determined by the researcher.