ProSci

IFI16 Antibody

Product Code:
 
PSI-15-315
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using IFI16 antibody (15-315) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat intestine using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
3 / 9
Immunohistochemistry of paraffin-embedded rat lung using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
4 / 9
Immunohistochemistry of paraffin-embedded rat liver using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
5 / 9
Immunohistochemistry of paraffin-embedded rat kidney using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
6 / 9
Immunohistochemistry of paraffin-embedded human kidney cancer using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
7 / 9
Immunohistochemistry of paraffin-embedded mouse lung using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using IFI16 antibody (15-315) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat intestine using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat lung using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IFI16 Antibody (15-315) at dilution of 1:100 (40x lens).

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PSI-15-315-50uL50uL£433.00
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Further Information

Additional Names:
IFNGIP1, PYHIN2, gamma-interferon-inducible protein 16, IFI16 beta isoform, interferon-gamma induced protein IFI 16, interferon-inducible myeloid differentiation transcriptional activator
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_005522.2).
NCBI Gene ID #:
3428
NCBI Official Name:
interferon gamma inducible protein 16
NCBI Official Symbol:
IFI16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 88kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Transcription
Swissprot #:
Q16666
User NOte:
Optimal dilutions for each application to be determined by the researcher.