ProSci

HTATSF1 Antibody

Product Code:
 
PSI-15-593
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using HTATSF1 antibody (15-593) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 5
Immunohistochemistry of paraffin-embedded human lung cancer using HTATSF1 antibody (15-593) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human gastric cancer using HTATSF1 antibody (15-593) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded mouse testis using HTATSF1 antibody (15-593) at dilution of 1:100 (40x lens).
5 / 5
Immunofluorescence analysis of C6 cells using HTATSF1 antibody (15-593) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HTATSF1 antibody (15-593) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human lung cancer using HTATSF1 antibody (15-593) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using HTATSF1 antibody (15-593) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using HTATSF1 antibody (15-593) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HTATSF1 antibody (15-593) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-15-593-50uL50uL£433.00
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Further Information

Additional Names:
HTATSF1, TATSF1, TAT-SF1, dJ196E23.2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
The protein encoded by this gene functions as a cofactor for the stimulation of transcriptional elongation by HIV-1 Tat, which binds to the HIV-1 promoter through Tat-TAR interaction. This protein may also serve as a dual-function factor to couple transcription and splicing and to facilitate their reciprocal activation. Alternatively spliced transcript variants have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human HTATSF1 (NP_055315.2).
NCBI Gene ID #:
27336
NCBI Official Name:
HIV-1 Tat specific factor 1
NCBI Official Symbol:
HTATSF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
O43719
User NOte:
Optimal dilutions for each application to be determined by the researcher.