ProSci

IDH3A Antibody

Product Code:
 
PSI-15-650
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using IDH3A antibody (15-650) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 5
Immunohistochemistry of paraffin-embedded rat kidney using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded human thyroid cancer using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded mouse kidney using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
❮❯

Western blot analysis of extracts of various cell lines, using IDH3A antibody (15-650) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded rat kidney using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human thyroid cancer using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using IDH3A antibody (15-650) at dilution of 1:100 (40x lens).

No additional charges, what you see is what you pay! *

CodeSizePrice
PSI-15-650-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT
How to order with us   Contact us
Stay in control of your spending. These prices have no additional charges, not even shipping!
* Rare exceptions are clearly labelled (only 0.14% of items!).
Multibuy discounts available! Contact us to find what you can save.
This product comes from: United States.
Typical lead time: 14-21 working days.
Contact us for more accurate information.
  • Further Information
  • Documents
  • Show All

Further Information

Additional Names:
IDH3A,
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 28-366 of human IDH3A (NP_005521.1).
NCBI Gene ID #:
3419
NCBI Official Name:
isocitrate dehydrogenase 3 (NAD+) alpha
NCBI Official Symbol:
IDH3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 40kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P50213
User NOte:
Optimal dilutions for each application to be determined by the researcher.