ProSci

S100A10 Antibody

Product Code:
 
PSI-15-656
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using S100A10 antibody (15-656) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 7
Immunohistochemistry of paraffin-embedded rat ovary using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse leydig cells using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using S100A10 antibody (15-656) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat ovary using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse leydig cells using S100A10 antibody (15-656) at dilution of 1:100 (40x lens).

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PSI-15-656-50uL50uL£433.00
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This product comes from: United States.
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Further Information

Additional Names:
S100A10, 42C, ANX2LG, Calpactin-1 light chain, Calpactin I light chain, CLP11, p11, p10, p10 protein, Protein S100-A10, ANX2L, Ca[1], CAL1L, Cellular ligand of annexin II, gp11
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-97 of human S100A10 (NP_002957.1).
NCBI Gene ID #:
6281
NCBI Official Name:
S100 calcium binding protein A10
NCBI Official Symbol:
S100A10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 11kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P60903
User NOte:
Optimal dilutions for each application to be determined by the researcher.