ProSci

PSMB7 Antibody

Product Code:
 
PSI-15-719
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using PSMB7 antibody (15-719) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 3
Immunofluorescence analysis of C6 cells using PSMB7 Polyclonal Antibody (15-719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of NIH-3T3 cells using PSMB7 Polyclonal Antibody (15-719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using PSMB7 antibody (15-719) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunofluorescence analysis of C6 cells using PSMB7 Polyclonal Antibody (15-719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH-3T3 cells using PSMB7 Polyclonal Antibody (15-719) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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PSI-15-719-50uL50uL£433.00
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Further Information

Additional Names:
PSMB7, proteasome subunit Z, proteasome subunit alpha, proteasome catalytic subunit 2, proteasome beta 7 subunit, proteasome (prosome, macropain) subunit, beta type, 7, multicatalytic endopeptidase complex chain Z, macropain chain Z, Z
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100
Background:
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. The encoded protein is a member of the proteasome B-type family, also known as the T1B family, and is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon, and proteolytic processing is required to generate a mature subunit. A pseudogene of this gene is located on the long arm of chromosome 14.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 44-200 of human PSMB7 (NP_002790.1).
NCBI Gene ID #:
5695
NCBI Official Name:
proteasome (prosome, macropain) subunit, beta type, 7
NCBI Official Symbol:
PSMB7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q99436
User NOte:
Optimal dilutions for each application to be determined by the researcher.