ProSci

CYP7B1 Antibody

Product Code:
 
PSI-18-145
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cells, using CYP7B1 antibody (18-145) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.
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Western blot analysis of extracts of various cells, using CYP7B1 antibody (18-145) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5min.

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PSI-18-145-50uL50uL£433.00
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Further Information

Additional Names:
CYP7B1, CP7B, cytochrome P450, family 7, subfamily B, polypeptide 1, oxysterol 7alpha-hydroxylase, subfamily VIIB (oxysterol 7 alpha-hydroxylase), CBAS3, SPG5A
Application Note:
WB: 1:500 - 1:1000
Background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway of extrahepatic tissues, which converts cholesterol to bile acids. This enzyme likely plays a minor role in total bile acid synthesis, but may also be involved in the development of atherosclerosis, neurosteroid metabolism and sex hormone synthesis.
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 200-300 of human CYP7B1 (NP_004811.1).
NCBI Gene ID #:
9420
NCBI Official Name:
cytochrome P450, family 7, subfamily B, polypeptide 1
NCBI Official Symbol:
CYP7B1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 58kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
O75881
User NOte:
Optimal dilutions for each application to be determined by the researcher.