ProSci

PPP1R1B Antibody

Product Code:
 
PSI-18-723
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of 293T cells, using PPP1R1B antibody (18-723) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 3s.
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Western blot analysis of extracts of 293T cells, using PPP1R1B antibody (18-723) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 3s.

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PSI-18-723-50uL50uL£433.00
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Further Information

Additional Names:
DARPP-32, DARPP32, protein phosphatase 1 regulatory subunit 1B, dopamine and cAMP-regulated neuronal phosphoprotein 32
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a bifunctional signal transduction molecule. Dopaminergic and glutamatergic receptor stimulation regulates its phosphorylation and function as a kinase or phosphatase inhibitor. As a target for dopamine, this gene may serve as a therapeutic target for neurologic and psychiatric disorders. Multiple transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-168 of human PPP1R1B (NP_001229393.1).
NCBI Gene ID #:
84152
NCBI Official Name:
protein phosphatase 1 regulatory inhibitor subunit 1B
NCBI Official Symbol:
PPP1R1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 32kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Obesity, Signal Transduction
Swissprot #:
Q9UD71
User NOte:
Optimal dilutions for each application to be determined by the researcher.