ProSci

MSI1 Antibody

Product Code:
 
PSI-18-736
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of A-549 cells, using MSI1 antibody (18-736) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
2 / 3
Immunohistochemistry of paraffin-embedded rat lung using MSI1 antibody (18-736) at dilution of 1:200 (40x lens).
3 / 3
Immunofluorescence analysis of U2OS cells using MSI1 antibody (18-736) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of A-549 cells, using MSI1 antibody (18-736) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit._Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat lung using MSI1 antibody (18-736) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using MSI1 antibody (18-736) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-18-736-50uL50uL£433.00
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Further Information

Additional Names:
MSI1, Musashi homolog 1 (Drosophila), Musashi RNA-binding protein 1, Musashi (Drosophila) homolog 1, Musashi1, Musashi 1, Musashi-1
Application Note:
WB: 1:500 - 1:1000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MSI1 (NP_002433.1).
NCBI Gene ID #:
4440
NCBI Official Name:
musashi RNA-binding protein 1
NCBI Official Symbol:
MSI1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Stem Cell
Swissprot #:
O43347
User NOte:
Optimal dilutions for each application to be determined by the researcher.