ProSci

KLKB1 Antibody

Product Code:
 
PSI-19-584
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 5
Western blot analysis of extracts of various cell lines, using KLKB1 antibody (19-584) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 5
Immunohistochemistry of paraffin-embedded rat kidney using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
3 / 5
Immunohistochemistry of paraffin-embedded rat brain using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human colon carcinoma using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
5 / 5
Immunohistochemistry of paraffin-embedded human liver cancer using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
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Western blot analysis of extracts of various cell lines, using KLKB1 antibody (19-584) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat kidney using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using KLKB1 antibody (19-584) at dilution of 1:200 (40x lens).

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PSI-19-584-50uL50uL£433.00
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Further Information

Additional Names:
KLKB1, KLK3, PPK
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. The encoded preproprotein present in plasma as a non-covalent complex with high molecular weight kininogen undergoes proteolytic processing mediated by activated coagulation factor XII to generate a disulfide-linked, heterodimeric serine protease comprised of heavy and light chains. Certain mutations in this gene cause prekallikrein deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 20-300 of human KLKB1 (NP_000883.2).
NCBI Gene ID #:
3818
NCBI Official Name:
kallikrein B, plasma (Fletcher factor) 1
NCBI Official Symbol:
KLKB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 71kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P03952
User NOte:
Optimal dilutions for each application to be determined by the researcher.