ProSci

POLD1 Antibody

Product Code:
 
PSI-19-587
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using POLD1 antibody (19-587) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using POLD1 antibody (19-587). Blue: DAPI for nuclear staining.
3 / 4
Immunofluorescence analysis of A549 cells using POLD1 antibody (19-587).
4 / 4
Immunofluorescence analysis of U2OS cells using POLD1 antibody (19-587). Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.
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Western blot analysis of extracts of various cell lines, using POLD1 antibody (19-587) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using POLD1 antibody (19-587). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cells using POLD1 antibody (19-587).
Immunofluorescence analysis of U2OS cells using POLD1 antibody (19-587). Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

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PSI-19-587-50uL50uL£433.00
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Further Information

Additional Names:
POLD1, CDC2, CDC2 homolog, POLD
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 808-1107 of human POLD1 (NP_002682.2).
NCBI Gene ID #:
5424
NCBI Official Name:
polymerase (DNA directed), delta 1, catalytic subunit
NCBI Official Symbol:
POLD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P28340
User NOte:
Optimal dilutions for each application to be determined by the researcher.