ProSci

HDGF Antibody

Product Code:
 
PSI-19-606
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using HDGF antibody (19-606) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using HDGF antibody (19-606) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using HDGF antibody (19-606) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using HDGF antibody (19-606) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat liver using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using HDGF Antibody (19-606) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using HDGF antibody (19-606) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using HDGF antibody (19-606) at dilution of 1:100. Blue: DAPI for nuclear staining.

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PSI-19-606-50uL50uL£433.00
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Further Information

Additional Names:
Hepatoma-derived growth factor, HDGF, High mobility group protein 1-like 2, HMG-1L2, HDGF, HMG1L2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human HDGF (NP_004485.1).
NCBI Gene ID #:
3068
NCBI Official Name:
Hepatoma-derived growth factor
NCBI Official Symbol:
HDGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Growth Factors
Swissprot #:
P51858
User NOte:
Optimal dilutions for each application to be determined by the researcher.