ProSci

C4BPB Antibody

Product Code:
 
PSI-22-158
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using C4BPB antibody (22-158) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 4
Immunohistochemistry of paraffin-embedded human stomach using C4BPB antibody (22-158) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded mouse kidney using C4BPB antibody (22-158) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of HeLa cells using C4BPB antibody (22-158). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using C4BPB antibody (22-158) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human stomach using C4BPB antibody (22-158) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using C4BPB antibody (22-158) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using C4BPB antibody (22-158). Blue: DAPI for nuclear staining.

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PSI-22-158-50uL50uL£433.00
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Further Information

Additional Names:
C4BPB, C4BP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes a member of a superfamily of proteins composed predominantly of tandemly arrayed short consensus repeats of approximately 60 amino acids. A single, unique beta-chain encoded by this gene assembles with seven identical alpha-chains into the predominant isoform of C4b-binding protein, a multimeric protein that controls activation of the complement cascade through the classical pathway. C4b-binding protein has a regulatory role in the coagulation system also, mediated through the beta-chain binding of protein S, a vitamin K-dependent protein that serves as a cofactor of activated protein C. The genes encoding both alpha and beta chains are located adjacent to each other on human chromosome 1 in the regulator of complement activation gene cluster. Alternative splicing gives rise to multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 18-251 of human C4BPB (NP_001017364.1).
NCBI Gene ID #:
725
NCBI Official Name:
complement component 4 binding protein, beta
NCBI Official Symbol:
C4BPB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 54kDa
Purification:
Affinity purification
Research Area:
Immunology, Innate Immunity
Swissprot #:
P20851
User NOte:
Optimal dilutions for each application to be determined by the researcher.