ProSci

TIMP4 Antibody

Product Code:
 
PSI-22-204
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using TIMP4 antibody (22-204) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of U2OS cells using TIMP4 antibody (22-204). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TIMP4 antibody (22-204) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using TIMP4 antibody (22-204). Blue: DAPI for nuclear staining.

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PSI-22-204-50uL50uL£433.00
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Further Information

Additional Names:
Metalloproteinase inhibitor 4, Tissue inhibitor of metalloproteinases 4, TIMP-4, TIMP4
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-224 of human TIMP4 (NP_003247.1).
NCBI Gene ID #:
7079
NCBI Official Name:
Metalloproteinase inhibitor 4
NCBI Official Symbol:
TIMP4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 25kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
Q99727
User NOte:
Optimal dilutions for each application to be determined by the researcher.