ProSci

TUFM Antibody

Product Code:
 
PSI-22-209
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 4
Western blot analysis of extracts of various cell lines, using TUFM antibody (22-209) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody (22-209) at dilution of 1:100 (40x lens).
3 / 4
Immunofluorescence analysis of MCF7 cells using TUFM antibody (22-209). Blue: DAPI for nuclear staining.
4 / 4
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TUFM antibody (22-209). Western blot was performed from the immunoprecipitate using TUFM antibody (22-209) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using TUFM antibody (22-209) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human esophageal cancer using TUFM antibody (22-209) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cells using TUFM antibody (22-209). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug TUFM antibody (22-209). Western blot was performed from the immunoprecipitate using TUFM antibody (22-209) at a dilition of 1:1000.

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PSI-22-209-50uL50uL£433.00
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Further Information

Additional Names:
TUFM, COXPD4, EF-TuMT, EFTU, P43
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:10 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes a protein which participates in protein translation in mitochondria. Mutations in this gene have been associated with combined oxidative phosphorylation deficiency resulting in lactic acidosis and fatal encephalopathy. A pseudogene has been identified on chromosome 17.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 186-455 of human TUFM (NP_003312.3).
NCBI Gene ID #:
7284
NCBI Official Name:
Tu translation elongation factor, mitochondrial
NCBI Official Symbol:
TUFM
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P49411
User NOte:
Optimal dilutions for each application to be determined by the researcher.