ProSci

SCG2 Antibody

Product Code:
 
PSI-22-211
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using SCG2 antibody (22-211) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 2
Immunofluorescence analysis of U2OS cells using SCG2 antibody (22-211). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using SCG2 antibody (22-211) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using SCG2 antibody (22-211). Blue: DAPI for nuclear staining.

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PSI-22-211-50uL50uL£433.00
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Further Information

Additional Names:
SCG2, secretogranin II, CHGC, EM66, SN, SgII, chromogranin-C, secretogranin-2, secretoneurin
Application Note:
WB: 1:500 - 1:2000

IF: 1:10 - 1:100
Background:
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. Studies in rodents suggest that the full-length protein, secretogranin II, is involved in the packaging or sorting of peptide hormones and neuropeptides into secretory vesicles. The full-length protein is cleaved to produce the active peptide secretoneurin, which exerts chemotaxic effects on specific cell types, and EM66, whose function is unknown.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 30-320 of human SCG2 (NP_003460.2).
NCBI Gene ID #:
7857
NCBI Official Name:
secretogranin II
NCBI Official Symbol:
SCG2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 65kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Chemokines & Cytokines, Immunology, Neuroscience
Swissprot #:
P13521
User NOte:
Optimal dilutions for each application to be determined by the researcher.