ProSci

MAPRE2 Antibody

Product Code:
 
PSI-22-378
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using MAPRE2 antibody (22-378) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunofluorescence analysis of HeLa cells using MAPRE2 antibody (22-378). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MAPRE2 antibody (22-378) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using MAPRE2 antibody (22-378). Blue: DAPI for nuclear staining.

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PSI-22-378-50uL50uL£433.00
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Further Information

Additional Names:
CSCSC2, EB1, EB2, RP1, microtubule-associated protein RP/EB family member 2, APC-binding protein EB1, APC-binding protein EB2, T-cell activation protein, EB1 family, end-binding protein 2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:100
Background:
The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. This protein is a microtubule-associated protein that is necessary for spindle symmetry during mitosis. It is thought to play a role in the tumorigenesis of colorectal cancers and the proliferative control of normal cells. Alternative splicing of this gene results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-327 of human MAPRE2 (NP_001137298.1).
NCBI Gene ID #:
10982
NCBI Official Name:
microtubule associated protein RP/EB family member 2
NCBI Official Symbol:
MAPRE2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 53kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q15555
User NOte:
Optimal dilutions for each application to be determined by the researcher.