ProSci

MPP2 Antibody

Product Code:
 
PSI-22-385
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of various cell lines, using MPP2 antibody (22-385) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of MCF7 cells using MPP2 antibody (22-385). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MPP2 antibody (22-385) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of MCF7 cells using MPP2 antibody (22-385). Blue: DAPI for nuclear staining.

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PSI-22-385-50uL50uL£433.00
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Further Information

Additional Names:
DLG2, MAGUK p55 subfamily member 2, discs large, homolog 2, membrane protein, palmitoylated 2 (MAGUK p55 subfamily member 2)
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human MPP2 (NP_005365.3).
NCBI Gene ID #:
4355
NCBI Official Name:
membrane palmitoylated protein 2
NCBI Official Symbol:
MPP2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 68kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q14168
User NOte:
Optimal dilutions for each application to be determined by the researcher.