ProSci

RPS4X Antibody

Product Code:
 
PSI-22-444
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 2
Western blot analysis of extracts of various cell lines, using RPS4X antibody (22-444) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
2 / 2
Immunohistochemistry of paraffin-embedded human kidney using RPS4X antibody (22-444) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of various cell lines, using RPS4X antibody (22-444) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human kidney using RPS4X antibody (22-444) at dilution of 1:100 (40x lens).

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PSI-22-444-50uL50uL£433.00
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Further Information

Additional Names:
40S ribosomal protein S4, X isoform, SCR10, Single copy abundant mRNA protein, RPS4X, CCG2, RPS4, SCAR
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Cytoplasmic ribosomes, organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes ribosomal protein S4, a component of the 40S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene and ribosomal protein S4, Y-linked (RPS4Y). The 2 isoforms encoded by these genes are not identical, but are functionally equivalent. Ribosomal protein S4 belongs to the S4E family of ribosomal proteins. This gene is not subject to X-inactivation. It has been suggested that haploinsufficiency of the ribosomal protein S4 genes plays a role in Turner syndrome; however, this hypothesis is controversial. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 66-263 of human RPS4X (NP_000998.1).
NCBI Gene ID #:
6191
NCBI Official Name:
40S ribosomal protein S4, X isoform
NCBI Official Symbol:
RPS4X
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 30kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62701
User NOte:
Optimal dilutions for each application to be determined by the researcher.