ProSci

ANXA11 Antibody

Product Code:
 
PSI-22-926
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
1 / 3
Western blot analysis of extracts of various cell lines, using ANXA11 antibody (22-926) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 3
Immunofluorescence analysis of A549 cells using ANXA11 antibody (22-926). Blue: DAPI for nuclear staining.
3 / 3
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody (22-926). Western blot was performed from the immunoprecipitate using ANXA11 antibody (22-926) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using ANXA11 antibody (22-926) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of A549 cells using ANXA11 antibody (22-926). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of Jurkat cells, using 3 ug ANXA11 antibody (22-926). Western blot was performed from the immunoprecipitate using ANXA11 antibody (22-926) at a dilition of 1:1000.

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PSI-22-926-50uL50uL£433.00
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Further Information

Additional Names:
ANXA11, ANX11, CAP50
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes a member of the annexin family, a group of calcium-dependent phospholipid-binding proteins. Annexins have unique N-terminal domains and conserved C-terminal domains, which contain calcium-dependent phospholipid-binding sites. The encoded protein is a 56-kD antigen recognized by sera from patients with various autoimmune diseases. Several transcript variants encoding two different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 276-505 of human ANXA11 (NP_001148.1).
NCBI Gene ID #:
311
NCBI Official Name:
annexin A11
NCBI Official Symbol:
ANXA11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P50995
User NOte:
Optimal dilutions for each application to be determined by the researcher.