ProSci

POLD4 Antibody

Product Code:
 
PSI-23-630
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
IgG
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Shipping:
 
Blue Ice or RT
Storage:
 
Store at -20°C. Avoid freeze / thaw cycles.
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Western blot analysis of extracts of MCF-7 cells, using POLD4 antibody (23-630) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of MCF-7 cells, using POLD4 antibody (23-630) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

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PSI-23-630-50uL50uL£433.00
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Further Information

Additional Names:
POLDS, p12, DNA polymerase delta subunit 4, DNA polymerase delta smallest subunit p12, polymerase (DNA) delta 4, accessory subunit, polymerase (DNA-directed), delta 4, accessory subunit
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-107 of human POLD4 (NP_066996.3).
NCBI Gene ID #:
57804
NCBI Official Name:
DNA polymerase delta 4, accessory subunit
NCBI Official Symbol:
POLD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
Q9HCU8
User NOte:
Optimal dilutions for each application to be determined by the researcher.