Nordic MuBio

Mouse anti Human IgG4 (pFc subclass specific) conjugated with Horseradish peroxidase

Product Code:
 
MAHu_IgG4_PO
Product Group:
 
Secondary Antibody
Supplier:
 
Nordic MuBio
Host Type:
 
Mouse
Antibody Isotype:
 
IgG1
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
NI 315 (HP 6206)
Regulatory Status:
 
RUO
Storage:
 
The lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C not refrozen and preferably used the same day.
 

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MAHu_IgG4_PO0.2 mg£573.00
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Further Information

Applications Description:
ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.
Background:
The reactivity of the antiserum is restricted to the subclass specific determinant on the pFc portion of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200; in ELISA from 1:500 upwards; in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.
Caution:
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
Formulation:
Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Label:
HRP
Product:
Purified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 ml sterile distilled water.
Product Form:
HRP
Source:
Highly purified monoclonal IgG4 isolated from pooled human serum
Specificity:
Peroxidase conjugated purified monoclonal Mouse antibody to Human IgG4, subclass specific. In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.