EGF Receptor / EGFR Antibody Cocktail

Flow cytometry: 0.5-1ug/million cells,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 2-4ug/ml for 30 min at RT

Optimal dilution of the EGFR antibody cocktail should be determined by the researcher.

1. Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37oC
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

This mAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Human EGF Receptor purified from A431 cells (31G7) and recombinant human protein (GFR1195) were used as the immunogen for the EGFR antibody cocktail.

This EGFR antibody cocktail is available for research use only.

Cell surface

Protein G affinity chromatography

Human

P00533