NSJ Bioreagents

EGF Receptor / EGFR Antibody

Product Code:
 
NSJ-V2106
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2a, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
B1D8
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Affinity Purification
  • Blocking
  • Fluorescence-activated cell sorting (FACS)
  • Immunoprecipitation (IP)
  • Inhibition Assay
Storage:
 
Store the EGF Receptor antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
 

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CodeSizePrice
NSJ-V2106-20UG20 ug£264.00
Quantity:
NSJ-V2106-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Affinity purification (order BSA/sodium azide-free format for coupling),Blocks ligand-induced activation of EGFR,Inhibits proliferation of A431 cells (order BSA/azide-free format),Arrests tumor growth in vivo (order BSA/azide-free format),Immunoprecipitation: 1-2ug/500ug protein lysate,Flow cytometry: 0.5-1ug/million cells
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.
Description:
This antibody reacts with the extracellular domain of EGF receptor and blocks the EGF/TGFa?induced activation. It also blocks tumor growth in vivo. It is excellent for purification of EGF receptor.?EGFR?is type I receptor tyrosine kinase with sequence homology to?ErbB2, -3 -4 or?HER2, -3 -4. It binds to Epidermal Growth Factor (EGF),?Transforming Growth Factor-a (TGFa), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Gene ID #:
1956
Immunogen:
Microsomes from A431 cells were used as the immunogen for this antibody.
Limitation:
This EGF Receptor antibody is available for research use only.
Localization:
Cell surface
Purity:
Protein G purified monoclonal antibody
Species Reactivity :
Human