Prolactin Receptor Antibody Cocktail

Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT

The optimal dilution of the Prolactin Receptor antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

It recognizes a protein of 70kDa, identified as prolactin receptor. Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. The initial step in its action is the binding to a specific membrane receptor (prolactin receptor), which belongs to the superfamily of class 1 cytokine receptors. The function of the prolactin receptor is mediated, at least in part, by two families of signaling molecules: Janus kinases and signal transducers and activators of transcription.

Purified

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Semi-purified human prolactin receptor (B6.2) and recombinant human protein (PRLR742) were used as the immunogens for this Prolactin Receptor antibody cocktail.

This Prolactin Receptor antibody cocktail is available for research use only.

Cell surface and cytoplasmic

Protein G affinity chromatography

Human

P16471