NSJ Bioreagents

CD68 Antibody

Product Code:
 
NSJ-V7390
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG2b, lambda
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
C68/2709
Regulatory Status:
 
RUO
Target Species:
 
Human
Application:
 
Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC staining of FFPE human tonsil with CD68 antibody (clone C68/2709). HIER: boil tissue sections in pH6 10mM citrate buffer, or pH 9 10mM Tris with 1mM EDTA, for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
Analysis of HuProt(TM) mi

IHC staining of FFPE human tonsil with CD68 antibody (clone C68/2709). HIER: boil tissue sections in pH6 10mM citrate buffer, or pH 9 10mM Tris with 1mM EDTA, for 10-20 min followed by cooling at RT for 20 min.~
Analysis of HuProt(TM) mi

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CodeSizePrice
NSJ-V7390-20UG20 ug£264.00
Quantity:
NSJ-V7390-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
Typical lead time: 10-14 working days.
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Further Information

Application Details :
Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the CD68 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Description:
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
A portion of amino acids 150-221 from the human protein was used as the immunogen for the CD68 antibody.
Limitation:
This CD68 antibody is available for research use only.
Localization:
Cytoplasmic, membrane
Microarray Validated?:
Yes
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P34810