NSJ Bioreagents

NSE Antibody / Neuron Specific Enolase

Product Code:
 
NSJ-V8361
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
ENO2/2706
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
 
Store the NSE antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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IHC staining of FFPE human neuroendocrine tumor with NSE antibody (clone ENO2/2706). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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IHC staining of FFPE human neuroendocrine tumor with NSE antibody (clone ENO2/2706). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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CodeSizePrice
NSJ-V8361-20UG20 ug£264.00
Quantity:
NSJ-V8361-100UG100 ug£534.00
Quantity:
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This product comes from: United States.
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Further Information

Application Details :
ELISA: Use Ab at 2-4ug/ml for coating; order Ab without BSA,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
Application Note:
Optimal dilution of the NSE antibody should be determined by the researcher.
Description:
This monoclonal antibody recognizes a protein of about 50kDa, which is identified as gamma-enolase. Three isoenzymes of enolases are identified, alpha, beta and gamma. Alpha-isoform is expressed in most tissues, whereas beta-form is expressed predominantly in muscle tissue whereas gamma-enolase is found only in nervous tissue. These isoforms exist as both homodimers and heterodimers, and they play a role in converting phosphoglyceric acid to phosphenolpyruvic acid in the glycolytic pathway. NSE-gamma is a useful marker to identify peripheral nerves and tumors of neuro-endocrine origins, such as pheochromocytomas.It it be usually employed in combination with other markers such as Synaptophysin,Chromogranin A, and Neurofilament.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Immunogen:
A portion of amino acids 416-433 from the human protein was used as the immunogen for the NSE antibody.
Limitation:
This NSE antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P09104