NSJ Bioreagents

EpCAM Antibody / Extracellular domain

Product Code:
 
NSJ-V7241
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Mouse
Antibody Isotype:
 
Mouse IgG1, kappa
Antibody Clonality:
 
Monoclonal
Antibody Clone:
 
EPM17-2
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the anti-EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
1 / 2
IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2). Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.~
2 / 2
Immunofluorescent
❮❯

IHC testing of human colorectal carcinoma with anti-EpCAM antibody (clone EPM17-2). Staining of FFPE tissue requires boiling sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.~
Immunofluorescent

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CodeSizePrice
NSJ-V7241-20UG20 ug£264.00
Quantity:
NSJ-V7241-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT (1)
Application Note:
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the anti-EpCAM antibody to be titered up or down for optimal performance.
Description:
EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Human partial recombinant protein (extracellular portion) was used as the immunogen for this anti-EpCAM antibody.
Limitation:
This anti-EpCAM antibody is available for research use only.
Localization:
Cell surface, cytoplasmic
Purity:
Protein G affinity chromatography
Species Reactivity :
Human
Uniprot #:
P16422