NSJ Bioreagents

Basic Cytokeratin Antibody

Product Code:
 
NSJ-V3812
Product Group:
 
Primary Antibodies
Supplier:
 
NSJ Bioreagents
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit IgG, kappa
Antibody Clonality:
 
Recombinant Antibody
Antibody Clone:
 
KRTH/2147R
Regulatory Status:
 
RUO
Target Species:
  • Human
  • Mouse
  • Rat
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store the recombinant Basic Cytokeratin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
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Immunofluorescent staining of MeOH-fixed human MCF7 cells with recombinant Basic Cytokeratin antibody (green, clone KRT18/834) and Reddot nuclear stain (red).~
2 / 2
IHC testing of FFPE human prostate cancer with recombinant Basic Cytokeratin
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Immunofluorescent staining of MeOH-fixed human MCF7 cells with recombinant Basic Cytokeratin antibody (green, clone KRT18/834) and Reddot nuclear stain (red).~
IHC testing of FFPE human prostate cancer with recombinant Basic Cytokeratin

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CodeSizePrice
NSJ-V3812-20UG20 ug£264.00
Quantity:
NSJ-V3812-100UG100 ug£534.00
Quantity:
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Further Information

Application Details :
Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2 ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 0.25-0.5ug/ml for 30 min at RT
Application Note:
Optimal dilution of the recombinant Basic Cytokeratin antibody should be determined by the researcher.
Description:
This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Format :
Purified
Formulation :
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Immunogen:
Solubilized keratin extract from human stratum corneum was used as the immunogen for the recombinant Basic Cytokeratin antibody.
Limitation:
This recombinant Basic Cytokeratin antibody is available for research use only.
Localization:
Cytoplasmic
Purity:
Protein A affinity chromatography
Species Reactivity :
Human, Mouse, Rat
Uniprot #:
Q01546